Improvement of a direct agglutination test for field studies of visceral leishmaniasis
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چکیده
منابع مشابه
Preparation and evaluation of latex agglutination technique for diagnosis and seroepidemiological studies of visceral leishmaniasis
in this study which has been carried out from 1996 until 1997 soluble latex agglutination antigen was prepared from L, donovani,sudan(s-1) by culturing in special media,harvesting,and later solubilizing by freezing and thawing.the antigen were futher prepared by high speed centrifugation.after measurement of the protein (lowry method) concentration of the antigen was set at 2.2 mg/ml,the antige...
متن کاملapplication of direct agglutination test (dat) for the diagnosis and seroepide-miological studies of visceral leishmaniasis in iran
visceral leishmaniasis (vl) is one of the most important parasitic diseases which is endemic in different parts of iran. serological studies were conducted by direct agglutination test (dat) on 12144 human serum samples, collected from four geographical zones of iran. sero prevalence, geographical distribution, clinical signs and symptoms for human visceral leishmaniasis based on dat for the pe...
متن کاملSeroepidemiological study of visceral Leishmaniasis (human infection) in East Myankooh area, in Lorestan Province by Direct Agglutination Test (DAT)
Background: Leishmaniasis is an infection caused by a protozoan of the genus Leishmania. This descriptive and cross-sectional study was done in order to determine the seroepidemiology of visceral leishmaniasis in East Myankooh area, in Lorestan Province during 2004 – 2005 time period. Materials and methods: This study was a descriptive, cross-sectional study. Multi stage sampling was performed...
متن کاملPerformance of Latex Agglutination Test (KAtex) In Diagnosis of Visceral Leishmaniasis in Iran
Background: Visceral leishmaniasis (VL) is an endemic disease in some parts of Iran. Many techniques have been used for diagnosis of VL, among which the urine based la-tex agglutination test (KAtex) is a promising one. Objective: To compare three diag-nostic tests of VL including KAtex, ELISA and Direct Agglutination Test (DAT) in VL patients and healthy controls in the south west of Iran. Meth...
متن کاملMulti-centre evaluation of repeatability and reproducibility of the direct agglutination test for visceral leishmaniasis.
OBJECTIVE To evaluate the repeatability and reproducibility of the serological direct agglutination test (DAT) for visceral leishmaniasis (VL) with aqueous antigen in a multi-centre study in VL-endemic areas in Sudan, Kenya and Nepal. METHODS Repeatability within each centre and reproducibility between the centres' results and an external reference laboratory (Belgium) was assessed on 1596 tr...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1988
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.26.7.1321-1325.1988